Question: What Would Happen If The Student Did Not Stop The Development Before The Solvent Reached The Top Of The Paper?

Which color was attracted to the mobile phase the most?

CaroteneBriefly explain the reasoning you used to identify this pigment.

Out of all the pigments, Carotene was the most attracted to the mobile phase.

Carotene was the most attracted because it was less charges or uncharged causing it to be more attracted to the mobile phase and be the furthest from the origin..

Why is it important to mark the solvent front quickly?

It is important to make sure the solvent is level and not at an angle so you do not get an angled solvent front. … As soon as the paper/plate is taken out, mark the solvent front with a pencil before the solvent evaporates and the front becomes impossible to see.

What happens if the spots are made too large when preparing a TLC plate for development?

What happens if spots are made too large when preparing a TLC plate for development? Why must the spots be above the level of development solvent in chamber? They will dissolve in the development solvent if they were submerged.

Why is it important to stop the chromatography before the solvent reaches the top of the paper?

It is important to stop the chromatograph before the solvent reaches the top of the paper because without knowing where the solvent reaches the top, you cannot calculate the Rf without measuring the distance from the origin to the solvent front.

What happens if the solvent front reaches the top of the plate?

However, if the solvent reaches the top of the plate, the chemicals continue to move up. This means that if left long enough, the chemicals will all merge together at the top of the plate, eliminating any separation that you could have seen on the plate.

Why can’t the solvent touch the pencil line?

If your start line is inside the solvent or equal to level of solvent it can cause error. The solvent gives extra energy to pull up the sample and the sample will also dissolve in the solvent. That’s why as precautionary measure start line should stay above the solvent in chromatography!

What would be the consequences of immersing the initial spots in your filter paper with your developing solvent?

If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself. … The solvent rises up the paper by CAPILLARITY. The solvent flows through the paper, over and past the mixture of substances.

Why is it important to remove the chromatography paper?

Why is it important to remove the chromatography paper from the beaker before the solvent front reaches the top of paper? Because you wouldn’t be able to measure the rf value unless you can measure the start to front of the solvent on the chromatography paper.

Which solvent system gave the best results?

Which solvent system gave the best results? The 75% hexane and 25% ethyl acetate gave the best results.

What might happen if a student used a pen to mark the baseline on the chromatography paper?

What might happen if a student used a pen to mark the baseline on the chromatography paper? The solution will move to the top and you would not be able to calculate the Rf value that is compared to the distance that a component traveled in the stationary phase reached by the solvent.

Why is it important to draw a line where the solvent stops before the paper dries out?

It is important that the solvent level is below the line with the spots on it. … The reason for covering the container is to make sure that the atmosphere in the beaker is saturated with solvent vapour. Saturating the atmosphere in the beaker with vapour stops the solvent from evaporating as it rises up the paper.

What would happen if the spots were sitting in the solvent?

Experiment 5: Thin Layer Chromatography and Melting Point 62 4) What would happen if your solvent level is above the level of the initial spots? Answer: The spots would dissolve into the reservoir of eluting solvent.

Why is a pen not used for chromatography?

Why is a pen not used for chromatography? Answer and Explanation: 1) Pen ink contains colored pigments. If we use the pen to mark on the chromatography paper then these pigments will also move along with the solvent and may interfere with the spots of our analyte. Hence we should not use the pen to mark on the paper.

Why is a pencil used to mark the position of the spots and not a pen?

A pencil was used to mark the chromatogram instead of a pen because the ink of the pen also contains ions that would cause inaccurate results in the chromatogram. 4. The cation samples are repeatedly spotted and dried on the chromatography paper to enhance the results so the colors can be visually seen later on.

What would happen if the solvent level is above the start line?

The solvent level has to be below the starting line of the TLC, otherwise the spots will dissolve away. … Non-polar solvents will force non-polar compounds to the top of the plate, because the compounds dissolve well and do not interact with the polar stationary phase.

What happens if the solvent line reaches the top of your TLC plate?

When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried, and the separated components of the mixture are visualized. If the compounds are colored, visualization is straightforward. Usually the compounds are not colored, so a UV lamp is used to visualize the plates.

What made the separation of the components of the ink possible?

Through the submersion of chromatography paper into water, any sample of ink can be separated into its respective cyan, magenta, and yellow components. The water causes the ink molecules to “travel” up the paper strip.

What information does the solvent front provide us with?

-Solvent front = distance up a TLC plate that the developing solvent travels. –used in determining the Rf value. -TLC chamber = medium in which the TLC is performed. -Spotting solvent = solvent used to dissolve the compound to be analyzed.