Quick Answer: What Would Be The Consequences Of Immersing The Initial Spots In Your Filter Paper With Your Developing Solvent?

What happens if a TLC plate is allowed to develop too long?

Chemicals move up a TLC plate along with the solvent being used to develop the plate.

This means that if left long enough, the chemicals will all merge together at the top of the plate, eliminating any separation that you could have seen on the plate..

Which color is most attracted to the solvent?

pink(b) I would say pink was the most attracted to the solvent because the majority of the color streak is pink, the whole middle section. Every ther color mostly branched from that pink color, and the pink showed up very bright and vivid.

What would happen if your solvent level is above the level of the initial spots?

Experiment 5: Thin Layer Chromatography and Melting Point 62 4) What would happen if your solvent level is above the level of the initial spots? Answer: The spots would dissolve into the reservoir of eluting solvent.

What would the result be if the spots are too concentrated in a TLC experiment?

Streaking: If the sample spot is too concentrated, the substance will travel up the stationary phase as a streak rather than a single separated spot. In other words, the solvent can not handle the concentrated sample and in result, moves as much of the substance as it can up the stationary phase.

Why is it important the ink dots are not submerged in the solvent?

It is important that the solvent level is below the line with the spots on it. … As the solvent slowly travels up the paper, the different components of the ink mixtures travel at different rates and the mixtures are separated into different colored spots.

Why must the solvent level be below the Coloured spots in chromatography?

The solvent level has to be below the starting line of the TLC, otherwise the spots will dissolve away. … Non-polar solvents will force non-polar compounds to the top of the plate, because the compounds dissolve well and do not interact with the polar stationary phase.

What has happened to the size of the starting material spots Why?

The size of the starting materials spots has decreased in size. This has happened as the material has moved up the TLC. How much time did it take to complete the reaction? It took about 34 mins for the reaction to complete.

How do you know when a TLC reaction is complete?

The reaction has gone to completion, when the TLC plate shows that there is no more starting material left in the reaction mixture.

What happens if the spots are made too large when preparing a TLC plate for development?

What happens if spots are made too large when preparing a TLC plate for development? Why must the spots be above the level of development solvent in chamber? They will dissolve in the development solvent if they were submerged.

Can water be used to separate the components of ink?

It can be useful in identifying unknown solutions as well. Through the submersion of chromatography paper into water, any sample of ink can be separated into its respective cyan, magenta, and yellow components. The water causes the ink molecules to “travel” up the paper strip.

Why must you be careful not to touch the filter paper with your fingers?

Ungloved human hands are made of proteins, which are made of amino acids. We are careful not to touch the filter paper so that we do not contaminate it with our own amino acids, which would later show up in the chromatography and throw off our data.

Why is it important to mark the solvent front immediately?

It’s important only if you want to determine retention/retardation factor absolutely for something you chromatographed. “Immediately” because it will soon vanish as the solvent evaporates. … Solvent front is the the furthest point reached by the solvent in chromatography.

What is the conclusion of paper chromatography?

Conclusion : Paper chromatography is a useful technique in the separation and identification of different plant pigments. In this technique, the mixture containing the pigments to be separated is first applied as a spot or a line to the paper about 1cm from the bottom edge of the paper.

Why does the filter paper need to be stapled so that the edges do not touch each other?

Staple the paper so that the ends of the paper do not touch each other, because if they do, it will affect the flow of the solvent at that point. … Use just enough solvent so that it will wet the bottom edge of the cylinder of paper, but will not be deep enough to touch the spots.

What happened to the ink in each solvent?

When you place chromatography paper into a solvent, the solvent begins to move up the paper. As the solvent rises, it dissolves the ink on the paper and separates the ink into its components. The farther the ink travels, the more it is attracted to the solvent.

Why must the spot applied to a chromatography plate be above the level of the developing solvent?

Used to separate volatile components of a mixture with low B.P. Why must the spot be applied to the TLC plate above the level of development solvent? … Low B.P. and thus more likely to evaporate off the plate before separation can occur.

Which solvent system gave the best results?

Which solvent system gave the best results? The 75% hexane and 25% ethyl acetate gave the best results.

Why is it very important in the amino acid chromatogram not to touch the paper with your fingers or hands?

The amino acids from your fingers will contaminate the paper and lead to erroneous results if it is touched on the “bottom”.

What will be the appearance of a TLC plate if a solvent of too low polarity is used for development?

If the solvent is of too low a polarity the components will not move enough, and again separation will not occur (Rf’s will be too small). … Note that the spotting solvent is simply used as a vehicle to transfer the material to be analyzed to the TLC plate. Once the transfer is made the spotting solvent evaporates.