- What is the difference between stationary and mobile phase?
- What pigment do you think is present in the highest concentration during the spring season when many leaves of deciduous plants are green?
- Which color is most attracted to the solvent?
- What color of light is not absorbed well by the carrot extract?
- Why is RF less than 1?
- What makes a good mobile phase?
- What does mobile phase mean?
- Which is the stationary phase in the dye part of this experiment?
- How do you separate colored ink?
- Why is ethanol better than water in chromatography?
- Is Red 40 more polar than blue?
- Which color has the greatest affinity for water?
- What is the mobile phase in chromatography?
- What is the mobile phase in high performance liquid chromatography?
- What is the most polar food dye?
- Why do colors separate in paper chromatography?
- Which dye AB or C is the least polar?
- Is red or blue more polar?
What is the difference between stationary and mobile phase?
In all chromatography there is a mobile phase and a stationary phase.
The stationary phase is the phase that doesn’t move and the mobile phase is the phase that does move.
The mobile phase moves through the stationary phase picking up the compounds to be tested..
What pigment do you think is present in the highest concentration during the spring season when many leaves of deciduous plants are green?
chlorophyllThe leaves of trees and other plants contain three main pigments: carotene, anthocyanin, and the photosynthetic pigment, chlorophyll, which captures the sun’s energy to make food for plants. As the most abundant pigment, chlorophyll is what gives leaves their green hue in spring and summer.
Which color is most attracted to the solvent?
pink(b) I would say pink was the most attracted to the solvent because the majority of the color streak is pink, the whole middle section. Every ther color mostly branched from that pink color, and the pink showed up very bright and vivid.
What color of light is not absorbed well by the carrot extract?
What wavelengths and colors of light are not absorbed well (reflected/transmitted) by the carrot extract (carotenoids)? Yellow, red, orange.
Why is RF less than 1?
Rf is a fraction. It is the ratio of how far a substance travels up the chromatography paper compared to the distance the solvent has travelled. This means that it must be less than 1.
What makes a good mobile phase?
A good mobile phase has the following characteristics. Compatible with the column packing/stationary phase and the sample. Gives good separation of the analytes of interest. Is of the highest purity.
What does mobile phase mean?
mobile phase (plural mobile phases) (chemistry) The fluid (liquid or gas) that flows through a chromatography system, moving the materials to be separated at different rates over the stationary phase.
Which is the stationary phase in the dye part of this experiment?
8 Cards in this SetWhy is it important to not spill the FD&C dyes on your skin or clothing?Because dyes will stain skin and clothingWhat will you use a the stationary phase in this experiment ?The strip of chromatography paper6 more rows
How do you separate colored ink?
Chromatography is a method for analyzing mixtures by separating them into the chemicals from which they are made. It can be used to separate mixtures like ink, blood, gasoline, and lipstick. In ink chromatography, you are separating the colored pigments that make up the color of the pen.
Why is ethanol better than water in chromatography?
The ink in the black marker is not soluble in water and therefore does not separate. … For more advanced students, explain that the alcohol is a better solvent for chromatographic separation [dissolving the ink] than water due to polar and non-polar interactions.)
Is Red 40 more polar than blue?
Red 40 dye is somewhat more polar than Blue 1 dye. … As the dilute alcohol solvent is passed through the column, the red dye, which is also slightly polar, is more attracted to the solvent than it is to the column.
Which color has the greatest affinity for water?
blueIn water, blue had a greater affinity for the solvent and pink had a greater affinity for the paper.
What is the mobile phase in chromatography?
Phases. Chromatography relies on two different ‘phases’: the mobile phase is the solvent that moves through the paper, carrying different substances with it. the stationary phase is contained on the paper and does not move through it.
What is the mobile phase in high performance liquid chromatography?
Sample carried by a moving gas stream of Helium or Nitrogen. High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).
What is the most polar food dye?
blue No. 1The most polar dye is blue No. 1. The leastpolar dye is red No. 3.
Why do colors separate in paper chromatography?
Often the colors that we see are a combination of the light reflected by a mixture of different-color molecules. … Different molecules run up the paper at different rates. As a result, components of the solution separate and, in this case, become visible as strips of color on the chromatography paper.
Which dye AB or C is the least polar?
Dye C is the least polar. The nonpolar solvent traveled very high in the container. Like dissolves like, so nonpolar dyes will be attracted to the nonpolar solvent, therefore following it up the container.
Is red or blue more polar?
Red 40 is a somewhat more polar molecule than is blue 1. This can be explained by the sizes of the molecules. Each molecule will have a similar amount of charge from ionization, but since blue 1 is a larger molecule the resulting charge will be spread out over a larger molecule.