Why Do Some Pigments Move Farther Than Others?

Which of the pigments is the least polar which is the most?

From this, we can deduce that carotenes are the least polar pigments (no polar groups), and xanthophylls are the most polar (two alcohol groups, one at each end of the molecule)..

What factors affect RF values in paper chromatography?

Here are some factors that affect the Rf value of paper chromatography.The solvent system.Composition of the mobile phase.The working temperature of the system.The quality of the paper used.The distance through which the solvent runs.The quality and nature of solvents used.The polarity of components.More items…•Apr 3, 2020

Which pigment traveled the shortest distance?

The least soluble pigment traveled the shortest distance, and that was the chlorophyll b. The chlorophyll a molecule was in the middle of the other two and showed an intermediate solubility.

Which pigment would you expect to move further on the chromatogram?

A small sample of plant pigment placed on chromatography paper travels up the paper due to capillary action. Beta carotene is carried the furthest because it is highly soluble in the solvent and because tit forms no hydrogen bonds with the chromatography paper fibers.

Why did some inks move a greater distance?

Why did some inks move a greater distance? The ink least readily absorbed by the paper would then travel the farthest from the starting mark. You can conclude from this information that the different pigments were absorbed at different rates.

What color is not absorbed by this pigment?

As shown in detail in the absorption spectra, chlorophyll absorbs light in the red (long wavelength) and the blue (short wavelength) regions of the visible light spectrum. Green light is not absorbed but reflected, making the plant appear green. Chlorophyll is found in the chloroplasts of plants.

How can you identify an unknown color pigment?

Paper chromatography is used as a qualitative analytical chemistry technique for identifying and separating colored mixtures like pigments. It is used in scientific studies to identify unknown organic and inorganic compounds from a mixture.

Why do pigments move the farthest?

Carotene moves the farthest because it is the most nonpolar of the pigments and it is attracted more strongly to the acetone-ligroin mixture (mobile phase) than to the paper. This stronger, nonbonded interaction with the mobile phase indicates that carotene is the most nonpolar pigment found in spinach chloroplasts.

Why do plant have different types of pigment?

Multiple pigments absorb different wavelengths of light, allowing the plant to capture the maximum amount of energy from the sun. … Multiple pigments allow the plants to have different colors of leaves, which allows them to capture the maximum amount of energy from the sun.

Can two compounds have the same Rf value?

The larger an Rf of a compound, the larger the distance it travels on the TLC plate. … If two substances have the same Rf value, they are likely (but not necessarily) the same compound. If they have different Rf values, they are definitely different compounds.

Why is RF less than 1?

Rf is a fraction. It is the ratio of how far a substance travels up the chromatography paper compared to the distance the solvent has travelled. This means that it must be less than 1.

Which pigment travels the fastest?

pigment caroteneThe pigment carotene, moved the furthest, therefore it traveled the fastest, but at a slightly slower rate than that of the acetone. This is evident from its distance from the solvent front; only 0.2 cm away. The pigment that moved the slowest, and therefore the smallest distance was chlorophyll b.

What color is Xanthophyll?

yellowXanthophyll (pronounced ZAN-tho-fill) – yellow. Carotene (pronounced CARE-a-teen) – gold, orange. Anthocyanin (pronounced an-tho-SIGH-a-nin) – red, violet, can also be bluish.

What pigment is the most soluble?

The orange colored band, made of the pigment called carotenoids. is the most soluble in alcohol, so it traveled the farthest. The yellow xanthophylls are the next most soluble, followed by the blue-green chlorophyll A. The least soluble pigment is the yellow green chlorophyll B.

Is chlorophyll a more polar than B?

The distinctions between the chlorophylls, which are more polar than β-carotene is slight: chlorophyll a has a methyl group (Y=CH3) in a position where chlorophyll b has an aldehyde (Y=CHO). This makes chlorophyll b slightly more polar than chlorophyll a.

Which color was most soluble in water?

orangea. The purple and orange colors are more soluble in water, because of the rate in which they traveled as the filter soaked up the water.

Does a higher Rf value mean more polar?

In general, the adsorptivity of compounds increases with increased polarity (i.e. the more polar the compound then the stronger it binds to the adsorbent). … Non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value).

Why do some pigments move further up the TLC plate than others?

Larger molecules take longer to move up the chromatography paper or TLC plate, whereas smaller molecules are more mobile. … Polar molecules will be more strongly attracted to polar solvents, and so would move further if a polar solvent was used as opposed to a non-polar solvent.