Why Does The Spot Have To Be Above The Solvent Level?

What would happen if the solvent level was above that of the dye spots on the paper at the start of the development?

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What would happen if the solvent level was above that of the dye spots on the paper at the start of the development.

Because the solvents used for chrom atography are volatile.

If it is not m arked im m ediately, there is a possibility that it m ay evaporate and then you would not be able to draw the solvent..

Why is it important to mark the solvent front immediately?

It’s important only if you want to determine retention/retardation factor absolutely for something you chromatographed. “Immediately” because it will soon vanish as the solvent evaporates. … Solvent front is the the furthest point reached by the solvent in chromatography.

Should the spot line be under or above the solvent level?

The solvent level has to be below the starting line of the TLC, otherwise the spots will dissolve away. The lower edge of the plate is then dipped in a solvent.

Which color was attracted to the mobile phase the most?

CaroteneBriefly explain the reasoning you used to identify this pigment. Out of all the pigments, Carotene was the most attracted to the mobile phase. Carotene was the most attracted because it was less charges or uncharged causing it to be more attracted to the mobile phase and be the furthest from the origin.

Which solvent resulted in the best separation of ink?

Water, alcohol or mixture of both are commonly used solvents. Colors are usually water soluble except permanent ink, which is soluble in organic solvents such as alcohol. Thus, water is the most suitable solvent for color separation.

How do you know when a TLC reaction is complete?

The reaction has gone to completion, when the TLC plate shows that there is no more starting material left in the reaction mixture.

Why is it important the ink dots are not submerged in the solvent?

It is important that the solvent level is below the line with the spots on it. … As the solvent slowly travels up the paper, the different components of the ink mixtures travel at different rates and the mixtures are separated into different colored spots.

What has happened to the size of the starting material spots Why?

The size of the starting materials spots has decreased in size. This has happened as the material has moved up the TLC. How much time did it take to complete the reaction? It took about 34 mins for the reaction to complete.

Can you think of any way of improving the separation between the different plant pigments?

The improved method may be applicable to all kinds of plant materials including algae, is easier than most other methods, and can lead to more successful results in separating these pigments by both thin-layer chromatography and paper chromatography.

What happened to the ink in each solvent?

When you place chromatography paper into a solvent, the solvent begins to move up the paper. As the solvent rises, it dissolves the ink on the paper and separates the ink into its components. The farther the ink travels, the more it is attracted to the solvent.

What will happen if your initial solvent level is above the baseline?

Experiment 5: Thin Layer Chromatography and Melting Point 62 4) What would happen if your solvent level is above the level of the initial spots? Answer: The spots would dissolve into the reservoir of eluting solvent. … You observe a melting point for this same compound isolated in your experiment as 177–181°C.

Why must the spots be above the solvent level?

It is important to keep the dye spots above the solvent level because if the spot is not below the solvent level, the molecules will not dissolve into the solvent and, then, would not be able to separate D.

What happens if the solvent line reaches the top of your TLC plate?

When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried, and the separated components of the mixture are visualized. If the compounds are colored, visualization is straightforward. Usually the compounds are not colored, so a UV lamp is used to visualize the plates.

Which solvent system gave the best results?

Which solvent system gave the best results? The 75% hexane and 25% ethyl acetate gave the best results.